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Image Search Results
Journal: Scientific Reports
Article Title: NMJ-Analyser identifies subtle early changes in mouse models of neuromuscular disease
doi: 10.1038/s41598-021-91094-6
Figure Lengend Snippet: Structural features of healthy NMJs in male C57BL/6J mice. ( a ) Confocal images of NMJs visualized using nerve terminal markers (2H3 + SV2, green) and motor endplate staining (alpha bungarotoxin, α-btx, red). Early-, mid- and late- mature NMJs display mono-innervated axonal input and pretzel-like shape. ( b ) Matrix correlation plot of nerve terminal and motor endplate parameters, and interaction between them measured in early-, mid- and late-mature NMJs. Positive and negative correlations are given by blue and red scales, respectively ( P value ≤ 5 × 10 −2 ). Datapoint showed non-normal distribution and spearman correlation tests were used. Empty circles indicate no significant P value was observed. ( c ) Module displaying NMJ morphological parameters measured in early-, mid- and late-mature NMJs. Early-mature NMJs are considered as baseline values. Morphological parameters were analysed using Wilcoxon signed-rank test. ( d ) PCA plots displaying variation between early-, mid- and late-mature NMJs. Scale bar = 20 μm.
Article Snippet: After overnight incubation, multiple washes were conducted with PBS-1X for at least 1 h. AlexaFluor-488 anti-mouse IgG secondary antibody (1/2000, Invitrogen) was added together with labelled
Techniques: Staining
Journal: Scientific Reports
Article Title: NMJ-Analyser identifies subtle early changes in mouse models of neuromuscular disease
doi: 10.1038/s41598-021-91094-6
Figure Lengend Snippet: Automatic analysis detects changes before manual assessment of NMJs in ALS and CMT2D mouse models. ( a ) Confocal images of NMJs visualized using nerve terminal markers (2H3 + SV2, green) and motor endplate staining (α-btx, red). Images correspond to SOD1 G93A/ + (3.5 months), Gars C201R/ + (1 month), FUS Δ14/ + (12 months) and TDP43 M323K/M323K (12 months). Arrows point to examples of full (dark orange), partial (white) and denervated NMJs (sky-blue). ( b ) NMJ innervation status evaluated by eye in whole-mount lumbrical muscles in ALS and CMT2D mouse models. 3.5-month old SOD1 G93A/ + and 1-month old Gars C201R/ + mice had a significant reduction in the percentage of fully innervated NMJs (* P value ≤ 0.05, Wilcoxon signed-rank test, two-tailed). ( c ) Module displaying NMJ morphological parameters measured in ALS and CMT2D strains using NMJ-Analyser. Each timepoint analysed was compared to their respective sex- and aged-matched WT littermates: * P value ≤ 5 × 10 −4 was considered significant. Percentage of innervation ± S.E.M. are plotted. Number of mice, WT and mutant: SOD1 G93A/ + , 1 month (5 + 5), 1.5 month (5 + 5), 3.5 months (4 + 9); Gars C201R/ + (5 + 5); FUS Δ14/ + , 3 months (4 + 4), 12 months (4 + 4); TDP43 M323K/M323K , 12 months (5 + 5). Scale bars = 20 μm.
Article Snippet: After overnight incubation, multiple washes were conducted with PBS-1X for at least 1 h. AlexaFluor-488 anti-mouse IgG secondary antibody (1/2000, Invitrogen) was added together with labelled
Techniques: Staining, Two Tailed Test, Mutagenesis
Journal: Scientific Reports
Article Title: NMJ-Analyser identifies subtle early changes in mouse models of neuromuscular disease
doi: 10.1038/s41598-021-91094-6
Figure Lengend Snippet: Performance of NMJ-Analyser and NMJ-Morph. ( a ) Confocal images of (i and ii) en-face and (iii) curved NMJs, visualized using nerve terminal markers (2H3 + SV2, green) and motor endplate staining (α-btx, red). Images correspond to Gars C201R/ + strain. ( b ) Correlation of nerve terminal and endplate output obtained using NMJ-Analyser and NMJ-Morph compared to a reference software. ( c ) Correlation of nerve terminal and endplate output obtained using NMJ-Analyser and NMJ-Morph, divided by genotype. ( b – c ) Datapoint showed non-normal distribution and spearman correlation tests were used. Green and red shading represent the confidence interval. ( d ) Boxplot of nerve terminal and endplate output using Volocity, NMJ-Analyser (μm 3 ) and NMJ-Morph (μm 2 ). ( e ) Boxplot of nerve terminal and endplate length (μm) and endplate compactness using NMJ-Analyser and NMJ-Morph. c-d outputs were analysed using a using Wilcoxon signed-rank test. Scale bars = 10 μm.
Article Snippet: After overnight incubation, multiple washes were conducted with PBS-1X for at least 1 h. AlexaFluor-488 anti-mouse IgG secondary antibody (1/2000, Invitrogen) was added together with labelled
Techniques: Staining, Software